/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-RNF5 Antibody
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Primary Antibodies  

Rabbit anti-RNF5 Antibody Affinity Purified

bethyl
Bethyl Laboratories

Catalog #

Validated
Target:

RNF5

Reactivity:

Human

Applications:

IP

,

WB

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

Between 1 and 50

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

Product Details

Specifications
Verified Reactivity

Human

Presumed Reactivity

Mouse,

Rat

Antigen Species

Human

Concentration
1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide


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Additional Product Information

RNF5 (ring finger protein 5) is a membrane-bound E3 ubiquitin ligase. It can regulate cell motility by targeting paxillin ubiquitination and altering the distribution and localization of paxillin in cytoplasm and cell focal adhesions [taken from NCBI Entrez Gene (Gene ID: 6048)].

6048

RNF5

ring finger protein 5

Q99942

E3 ubiquitin-protein ligase RNF5

Alternate Names

E3 ubiquitin-protein ligase RNF5; HsRma1; protein G16; ram1 homolog; RING finger protein 5; ring finger protein 5, E3 ubiquitin protein ligase; RING5; RING-type E3 ubiquitin transferase RNF5; RMA1

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.