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Rabbit anti-eIF4AIII/EIF4A3 Antibody

Bethyl Laboratories®
Bethyl Laboratories®

Catalog #

Validated
Citations (7)
Target:

eIF4AIII/EIF4A3

Reactivity:

Human

,

Mouse

Applications:

IHC

,

IP

,

WB

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

between 125 and 175

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (200 µg/ml)

10 µl (200 µg/ml)

Product Details

Specifications
Verified Reactivity

Mouse,

Human

Presumed Reactivity

Chicken,

Pig,

X_laevis,

X_tropicalis,

Zebrafish,

Bovine,

Rat

Antigen Species

Human

Concentration
200 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide


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Additional Product Information

Eukaryotic initiation factor 4A-III is a member of the DEAD box family of proteins that possesses several conserved motifs which include the highly conserved DEAD (Asp-Glu-Ala-Asp) amino acid sequence motif. The major activity of DEAD box proteins is to function as ATP-dependent RNA helicases. eIF4AIII is an ATP-dependent helicase and a component of a splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs.

9775

EIF4A3

eukaryotic translation initiation factor 4A3

P38919

Eukaryotic initiation factor 4A-III

Alternate Names

ATP-dependent RNA helicase DDX48; ATP-dependent RNA helicase eIF4A-3; DDX48; DEAD (Asp-Glu-Ala-Asp) box polypeptide 48; DEAD box protein 48; eIF4AIII; eIF-4A-III; eIF4A-III; eukaryotic initiation factor 4A-III; eukaryotic initiation factor 4A-like NUK-34; eukaryotic translation initiation factor 4A; Eukaryotic translation initiation factor 4A isoform 3; Fal1; hNMP 265; MUK34; NMP 265; NMP265; nuclear matrix protein 265; NUK34; RCPS

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.