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Primary Antibodies  

Rabbit anti-MED15 Antibody Affinity Purified

Bethyl Laboratories®
Bethyl Laboratories®

Catalog #

Validated
Citations (7)
Target:

MED15

Reactivity:

Human

Applications:

IHC

,

IP

,

WB

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

between 450 and 500

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (200 µg/ml)

10 µl (200 µg/ml)

Product Details

Specifications
Verified Reactivity

Human

Presumed Reactivity

Mouse

Antigen Species

Human

Concentration
200 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide


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Additional Product Information

Mediator complex subunit 15 is a component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. MED15 is required for cholesterol-dependent gene regulation. It also positively regulates the Nodal signaling pathway. [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q96RN5].

51586

MED15

mediator complex subunit 15

Q96RN5

Mediator of RNA polymerase II transcription subunit 15

Alternate Names

activator-recruited cofactor 105 kDa component; activator-recruited cofactor, 105-kD; ARC105; CAG7A; CTG repeat protein 7a; CTG7A; Mediator complex subunit 15; mediator of RNA polymerase II transcription subunit 15; PC2 (positive cofactor 2, multiprotein complex) glutamine/Q-rich-associated protein; PC2 glutamine/Q-rich-associated protein; PC2-glutamine-rich-associated protein; PCQAP; Positive cofactor 2 glutamine/Q-rich-associated protein; positive cofactor 2, glutamine/Q-rich-associated protein; TIG1; TIG-1; TNRC7; TPA inducible gene-1; TPA inducible protein; TPA-inducible gene 1 protein; trinucleotide repeat containing 7; trinucleotide repeat-containing gene 7 protein

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.