/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-ERCC5/XPG Antibody
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Primary Antibodies  

Rabbit anti-ERCC5/XPG Antibody, Affinity Purified

bethyl
Bethyl Laboratories

Catalog #

Validated
Citations (14)
Target:

ERCC5/XPG

Reactivity:

Human

Applications:

IHC

,

IP

,

WB

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

Between 650 and 700

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

Product Details

Specifications
Verified Reactivity

Human

Antigen Species

Human

Concentration
1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide


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Production Procedures

Antibody was affinity purified using an epitope specific to ERCC5/XPG immobilized on solid support.

The epitope recognized by A301-484A-T maps to a region between residue 650 and 700 of human excision repair cross-complementing rodent repair deficiency, complementation group 5 (xeroderma pigmentosum, complementation group G (Cockayne syndrome)) using the numbering given in entry NP_000114.2 (GeneID 2073).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Antibody was affinity purified using an epitope specific to ERCC5/XPG immobilized on solid support.

The epitope recognized by A301-484A maps to a region between residue 650 and 700 of human excision repair cross-complementing rodent repair deficiency, complementation group 5 (xeroderma pigmentosum, complementation group G (Cockayne syndrome)) using the numbering given in entry NP_000114.2 (GeneID 2073).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Additional Product Information

ERCC5 is a single-stranded DNA endonuclease involved in DNA excision repair. ERCC5 is an important component of the nucleotide excision repair (NER) pathway which is critical to the repair of DNA lesions caused by exposure to UV light. Defects in ERCC5 are the cause of xeroderma pigmentosum, an autosomal recessive disease characterized by a hypersensitivity to sunlight and a high predisposition to cancer on UV-exposed areas.

2073

ERCC5

ERCC excision repair 5, endonuclease

P28715

DNA repair protein complementing XP-G cells

Alternate Names

COFS3; DNA excision repair protein ERCC-5; DNA repair protein complementing XP-G cells; ERCC5-201; ERCM2; excision repair cross-complementation group 5; excision repair cross-complementing rodent repair deficiency, complementation group 5; UVDR; Xeroderma pigmentosum group G-complementing protein; xeroderma pigmentosum, complementation group G; XPG; XPGC; XPG-complementing protein

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.