/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-FOG1/ZFPM1 Antibody
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Primary Antibodies  

Rabbit anti-FOG1/ZFPM1 Antibody Affinity Purified

bethyl
Bethyl Laboratories

Catalog #

Validated
Target:

FOG1/ZFPM1

Reactivity:

Human

Applications:

IHC

,

IP

,

WB

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

Between 175 and 225

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (200 µg/ml)

10 µl (200 µg/ml)

Product Details

Specifications
Verified Reactivity

Human

Antigen Species

Human

Concentration
200 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide


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Production Procedures

Antibody was affinity purified using an epitope specific to FOG1/ZFPM1 immobilized on solid support.

The epitope recognized by A301-431A maps to a region between residue 175 and 225 of human friend of GATA-1 (zinc finger protein, multitype 1) using the numbering given in entry NP_722520.2 (GeneID 161882).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Antibody was affinity purified using an epitope specific to FOG1/ZFPM1 immobilized on solid support.

The epitope recognized by A301-431A-T maps to a region between residue 175 and 225 of human friend of GATA-1 (zinc finger protein, multitype 1) using the numbering given in entry NP_722520.2 (GeneID 161882).

Additional Product Information

FOG1/ZFPM1 was identified in a yeast two-hybrid screen for GATA-1 interacting proteins. GATA-1 is zinc finger transcription factor whose expression is restricted to hematopoietic cells and plays and essential role in erythroid development. FOG1/ZFPM1 is highly expressed in erythroid cells and megakaryocytes and appears to play both a GATA-1-dependent and -independent role in erythropoiesis and megakaryopoiesis. FOG1/ZFPM1 associates with GATA-1 as well as GATA-2 and GATA-3 as a heterodimer to activate or repress transcription.

161882

ZFPM1

zinc finger protein, FOG family member 1

Q8IX07

Zinc finger protein ZFPM1

Alternate Names

FOG; FOG1; FOG-1; friend of GATA 1; friend of GATA protein 1; ZC2HC11A; zinc finger protein 89A; Zinc finger protein multitype 1; zinc finger protein ZFPM1; zinc finger protein, multitype 1; ZNF408; ZNF89A

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.