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Rabbit anti-EPB41L2/4.1G Antibody Affinity Purified

bethyl
Bethyl Laboratories

Catalog #

Validated
Target:

EPB41L2/4.1G

Reactivity:

Human

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

Between 100 and 150

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (200 µg/ml)

10 µl (200 µg/ml)

Product Details

Specifications
Verified Reactivity

Human

Antigen Species

Human

Concentration
200 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide


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Production Procedures

Antibody was affinity purified using an epitope specific to EPB41L2/4. 1G immobilized on solid support.

The epitope recognized by A301-424A maps to a region between residue 100 and 150 of human erythrocyte membrane protein band 4.1-like 2 (Generally expressed protein 4.1) using the numbering given in entry NP_001422.1 (GeneID 2037).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Antibody was affinity purified using an epitope specific to EPB41L2/4. 1G immobilized on solid support.

The epitope recognized by A301-424A-T maps to a region between residue 100 and 150 of human erythrocyte membrane protein band 4.1-like 2 (Generally expressed protein 4.1) using the numbering given in entry NP_001422.1 (GeneID 2037).

Additional Product Information

EPB41L2/4.1G is a member of the protein 4.1 family which includes erythroid protein 4.1 (4.1R), 4.1N (neuronal), 4.1B (brain) and 4.1G (general). The protein 4.1 family members are defined by the presence of a FERM, SAB, and CTD domain and play a role in cell membrane and cytoskeletal structural organization. EPB41L2/4.1G is widely expressed and localizes to the cytosol, perinuclear area, and centrosomes.

2037

EPB41L2

erythrocyte membrane protein band 4.1 like 2

O43491

Band 4.1-like protein 2

Alternate Names

4.1G; 4.1-G; band 4.1-like protein 2; erythrocyte membrane protein band 4.1 like-protein 2; generally expressed protein 4.1

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.