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Rabbit anti-CCDC16 Antibody Affinity Purified

bethyl
Bethyl Laboratories

Catalog #

Validated
Target:

CCDC16

Reactivity:

Human

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

Between 322 and C-terminus

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (200 µg/ml)

10 µl (200 µg/ml)

Product Details

Specifications
Verified Reactivity

Human

Presumed Reactivity

Mouse,

Rat

Antigen Species

Human

Concentration
200 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide


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Production Procedures

Antibody was affinity purified using an epitope specific to CCDC16 immobilized on solid support.

The epitope recognized by A301-419A maps to a region between residue 322 and 372 of human coiled-coil domain containing 16 using the numbering given in entry NP_443089.3 (GeneID 91603).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Antibody was affinity purified using an epitope specific to CCDC16 immobilized on solid support.

The epitope recognized by A301-419A-T maps to a region between residue 322 and 372 of human coiled-coil domain containing 16 using the numbering given in entry NP_443089.3 (GeneID 91603).

Additional Product Information

CCDC16 (coiled-coil domain containing 16) is a U1-like C2H2-type zinc finger protein whose function has not been characterized. Coiled-coil proteins exhibit tertiary structures made up of a number of highly organized alpha helices wound around each other to mediate oligomerization.

91603

ZNF830

zinc finger protein 830

Q96NB3

Zinc finger protein 830

Alternate Names

CCDC16; coiled-coil domain containing 16; coiled-coil domain-containing protein 16; hypothetical protein MGC20398; OMCG1; orphan maintenance of genome 1; zinc finger protein 830

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.