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Rabbit anti-CENTD2/ARAP1 Antibody Affinity Purified

bethyl
Bethyl Laboratories

Catalog #

Validated
Target:

CENTD2/ARAP1

Reactivity:

Human

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

Between 1400 and C-terminus

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

Product Details

Specifications
Verified Reactivity

Human

Antigen Species

Human

Concentration
1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide


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Production Procedures

Antibody was affinity purified using an epitope specific to CENTD2/ARAP1 immobilized on solid support.

The epitope recognized by A301-413A maps to a region between residue 1160 and 1210 of human centaurin, delta 2 (ankyrin repeat, and pleckstrin homology domains-containing protein 1) using the numbering given in entry NP_056057.1 (GeneID 116985).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Antibody was affinity purified using an epitope specific to CENTD2/ARAP1 immobilized on solid support.

The epitope recognized by A301-413A-T maps to a region between residue 1160 and 1210 of human centaurin, delta 2 (ankyrin repeat, and pleckstrin homology domains-containing protein 1) using the numbering given in entry NP_056057.1 (GeneID 116985).

Additional Product Information

CENTD2/ARAP1 is an adapter protein that functions as an Arf 1 and Rho GTPase-activation protein. CENTD2/ARAP1 associates with the Golgi and appears to play a role in cytoskeleton remodeling as well as membrane receptor recycling and trafficking.

116985

ARAP1

ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 1

Q96P48

Arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 1

Alternate Names

arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 1; ARF-GAP, RHO-GAP, ankyrin repeat, and pleckstrin homology domains-containing protein 1; centaurin-delta-2; CENTD2; cnt-d2

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.