/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-WRNIP1 Antibody
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Primary Antibodies  

Rabbit anti-WRNIP1 Antibody Affinity Purified

bethyl
Bethyl Laboratories

Catalog #

Validated
Citations (1)
Target:

WRNIP1

Reactivity:

Human

Applications:

IHC

,

IP

,

WB

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

Between 400 and 450

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

Product Details

Specifications
Verified Reactivity

Human

Presumed Reactivity

Mouse,

Rat

Antigen Species

Human

Concentration
1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide


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Production Procedures

Antibody was affinity purified using an epitope specific to WRNIP1 immobilized on solid support.

The epitope recognized by A301-389A maps to a region between residue 400 and 450 of human Werner helicase interacting protein 1 using the numbering given in entry NP_064520.2 (GeneID 56897).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Antibody was affinity purified using an epitope specific to WRNIP1 immobilized on solid support.

The epitope recognized by A301-389A-T maps to a region between residue 400 and 450 of human Werner helicase interacting protein 1 using the numbering given in entry NP_064520.2 (GeneID 56897).

Additional Product Information

WRNIP1 (Werner helicase interacting protein 1) was identified as a protein that interacts with the N-terminal region of Werner protein (WRN). WRN is a RecQ DNA helicase important to DNA repair and is the causative gene of Werner syndrome (WS), an autosomal recessive disease characterized by premature aging. WRNIP1 has been shown to interact with DNA polymerase delta (pol delta) and play a role in pol delta-mediated DNA synthesis. Additionally, WRNIP1 has been shown to interact with polyubiquitin chains indicating a link between ubiquitination and the DNA repair pathway.

56897

WRNIP1

Werner helicase interacting protein 1

Q96S55

ATPase WRNIP1

Alternate Names

ATPase WRNIP1; bA420G6.2; putative helicase RUVBL; Werner helicase-interacting protein 1; WHIP

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.