/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-AKAP2 Antibody
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Primary Antibodies  

Rabbit anti-AKAP2 Antibody Affinity Purified

bethyl
Bethyl Laboratories

Catalog #

Validated
Target:

AKAP2

Reactivity:

Human

Applications:

IP

,

WB

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

Between 325 and 375

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (200 µg/ml)

10 µl (200 µg/ml)

Product Details

Specifications
Verified Reactivity

Human

Antigen Species

Human

Concentration
200 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide


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Production Procedures

Antibody was affinity purified using an epitope specific to AKAP2 immobilized on solid support.

The epitope recognized by A301-363A maps to a region between residue 75 and 125 of human A kinase (PRKA) anchor protein 2 using the numbering given in entry Q9Y2D5.2 (GeneID 11217).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Antibody was affinity purified using an epitope specific to AKAP2 immobilized on solid support.

The epitope recognized by A301-363A-T maps to a region between residue 75 and 125 of human A kinase (PRKA) anchor protein 2 using the numbering given in entry Q9Y2D5.2 (GeneID 11217).

Additional Product Information

AKAP2 (A-kinase anchoring protein 2) was identified in a yeast two-hybrid screen as a protein that interacts with protein kinase A (PKA). AKAP2 functions to direct the subcellular localization of PKA and may also function as a link for cytoskeletal signaling complexes. Structurally, AKAP2 contains two tandem RGS domains, a PKA-binding motif, and a PDZ (PSD95/Dlg/ZO1)-binding motif.

11217

AKAP2

A-kinase anchoring protein 2

Q9Y2D5

A-kinase anchor protein 2

Alternate Names

A kinase (PRKA) anchor protein 2; AKAP-2; AKAPKL; AKAP-KL; A-kinase anchor protein 2; MISP2; PRKA2; protein kinase A anchoring protein 2; protein kinase A2; Protein kinase A-anchoring protein 2

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.