/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-RCOR3 Antibody
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Primary Antibodies  

Rabbit anti-RCOR3 Antibody Affinity Purified

bethyl
Bethyl Laboratories

Catalog #

Validated
Citations (1)
Target:

RCOR3

Reactivity:

Human

,

Mouse

Applications:

ICC-IF

,

IHC

,

IHC-IF

,

IP

,

WB

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

between 445 and 495

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (200 µg/ml)

10 µl (200 µg/ml)

Product Details

Specifications
Verified Reactivity

Mouse,

Human

Antigen Species

Human

Concentration
200 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide


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Production Procedures

Antibody was affinity purified using an epitope specific to RCOR3 immobilized on solid support.

The epitope recognized by A301-273A maps to a region between residue 445 and 495 of human REST corepressor 3 using the numbering given in entry NP_060724.1 (GeneID 55758).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Antibody was affinity purified using an epitope specific to RCOR3 immobilized on solid support.

The epitope recognized by A301-273A-T maps to a region between residue 445 and 495 of human REST corepressor 3 using the numbering given in entry NP_060724.1 (GeneID 55758).

Additional Product Information

RCOR3 is a member of the coREST family that functions as a co-repressor for REST (RE1-silencing transcription factor) to regulate neuronal gene expression and neuronal stem cell fate. The coREST family includes RCOR1, also known as coREST, RCOR2, and RCOR3. REST complexes regulate gene expression by modifying chromatin to create a repressive epigenetic environment.

55758

RCOR3

REST corepressor 3

Q9P2K3

REST corepressor 3

Alternate Names

REST corepressor 3

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.