/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-ANKRD28/PITK Antibody
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Primary Antibodies  

Rabbit anti-ANKRD28/PITK Antibody Affinity Purified

bethyl
Bethyl Laboratories

Catalog #

Validated
Citations (3)
Target:

ANKRD28/PITK

Reactivity:

Human

Applications:

IHC

,

IP

,

WB

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

Between 1003 and C-terminus

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (200 µg/ml)

10 µl (200 µg/ml)

Product Details

Specifications
Verified Reactivity

Human

Antigen Species

Human

Concentration
200 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide


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Additional Product Information

ANKRD28/PITK is a regulatory subunit of protein phosphatase-1 (PP1) that targets the PP1 catalytic subunit to nuclear foci to dephosphorylate and modulate the transcriptional activity of heterogeneous nuclear ribonucleoprotein K (hnRNPK). ANKRD28/PITK has been shown to be phosphorylated and regulated by calcium/calmodulin-dependent kinase II-delta (CaMKIIdelta) and glycogen synthase kinase-3 (GSK3).

23243

ANKRD28

ankyrin repeat domain 28

O15084

Serine/threonine-protein phosphatase 6 regulatory ankyrin repeat subunit A

Alternate Names

ankyrin repeat domain-containing protein 28; phosphatase interactor targeting K protein; phosphatase interactor targeting protein hnRNP K; PITK; PP6-ARS-A; PPP1R65; protein phosphatase 1, regulatory subunit 65; protein phosphatase 6 ankyrin repeat subunit A; serine/threonine-protein phosphatase 6 regulatory ankyrin repeat subunit A; serine/threonine-protein phosphatase 6 regulatory subunit ARS-A

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.