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Goat anti-Rabbit IgG Heavy and Light Chain Highly Cross-Adsorbed Antibody HRP Conjugated
Bethyl Laboratories
Catalog #
Target:
Rabbit IgG Heavy and Light Chain
Reactivity:
Rabbit
Applications:
Flow Cyt
,ICC
,IF
,IHC
Host:
Goat
Clonality:
Polyclonal
Format:
Whole IgG
Isotype:
IgG
Conjugate:
FITC
,HRP
Purity:
Antigen Affinity Purified
FITC (1 mg)
HRP (1 mg)
Product Details
Specifications
Verified Reactivity
Rabbit
Antigen Species
Rabbit
Minimum Reactivity Species
Human, Mouse
Concentration
1 mg/ml
Fluorophore/Protein
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Phosphate Buffered Saline (PBS) containing 0.2% BSA and 0.05% Pro-Clean 400
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Phosphate Buffered Saline (PBS) containing 0.2% BSA and 0.09% Sodium Azide
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Production Procedures
Antiserum was cross adsorbed using human and mouse immunosorbents to remove cross reactive antibodies. The antibody to rabbit IgG was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to fluorescein isothiocyanate (FITC).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
This antibody reacts specifically with rabbit IgG in immunohistochemistry and immunocytochemistry fluorescence assays. Non-specific binding to human and mouse tissue is negligible.
Additional Product Information
Anti-heavy- and light-chain antibodies are designed to react with the whole intact Ig molecule. Found in all body fluids and a primary means of protection against infection, an anti-H+L IgG antibody allows for some potential cross-reactivity to other Ig molecules and IgG molecules from other closely-related species.
Applications
