Detection of Human PAF49/CAST by ICC-IF. Sample: Formaldehyde-fixed asynchronous HeLa cells. Antibody: Affinity purified rabbit anti-PAF49/CAST (Cat. No. IHC-00474) used at a dilution of 1:125. Detection: Red-fluorescent goat anti-rabbit IgG highly cross-adsorbed Antibody Hilyte Plus 555 (A120-501E) used at a dilution of 1:100.
Detection of Human KIF14 by ICC-IF. Sample: Formaldehyde-fixed asynchronous HeLa cells. Antibody: Affinity purified rabbit anti-KIF14 (Cat. No. IHC-00475) used at a dilution of 1:100. Detection: Red-fluorescent goat anti-rabbit IgG highly cross-adsorbed Antibody Hilyte Plus 555 (A120-501E) used at a dilution of 1:100.
Detection of Human Gemin4 by IHC. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-Gemin4 (Cat. No. IHC-00481) used at a dilution of 1:250. Detection: DAB staining using Immunohistochemistry Accessory Kit (Cat. No. IHC-101). Epitope Retrieval Buffer-High pH (IHC-101J) was substituted for Epitope Retrieval Buffer-Reduced pH.
Immunohistochemistry Accessory Kit (Cat. No. IHC-101) provides the reagents and method for sensitive, reliable and economical immunohistochemical staining. This Kit is designed for immunostaining formalin-fixed, paraffin-embedded tissue sections with primary antibodies made in rabbit. It contains all the reagents required for the immunostaining procedure and will stain 250 slides.
Detection of Human SA1 by WB and IP. Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Antibodies: SA1 antibody A302-579A used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. SA1 was also immunoprecipitated by SA1 antibody A302-578A, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).
Detection of Human SA2 by WB and IP. Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Antibodies: SA2 antibody A302-580A used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 3 mcg/mg lysate. SA2 was also immunoprecipitated by rabbit anti-SA2 antibody A302-581A, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 1 second (B).
Detection of Human MCM7 by WB and IP. Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Antibodies: MCM7 antibody A302-584A used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. MCM7 was also immunoprecipitated by rabbit anti-MCM7 antibodies A302-585A and A302-586A, which recognize downstream epitopes. Detection: Chemiluminescence with exposure time of 30 seconds (A) and 3 seconds (B).
ReliaBLOT® IP/Western Blot Reagents and Procedures (patent pending) provide an improved method for the detection of immunoprecipitated proteins assayed via Western Blot. It seamlessly integrates into existing IP/WB Protocols with three simple changes. It is optimized for antibodies made in rabbit.
