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Primary Antibodies  

Rabbit anti-HNF1-alpha Antibody

Bethyl Laboratories®
Bethyl Laboratories®

Catalog #

Validated
Target:

HNF1-alpha

Reactivity:

Human

Applications:

IHC

,

IP

,

WB

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

Between 550 and 600

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

Product Details

Specifications
Verified Reactivity

Human

Antigen Species

Human

Concentration
1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide


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Additional Product Information

Hepatocyte nuclear factor 1-alpha (HNF1-Alpha) is a transcription factor required for the expression of several liver-specific genes. HNF1-Alpha functions as a homodimer and binds to the inverted palindrome 5'-GTTAATNATTAAC-3'. Defects in the HNF1A gene are a cause of maturity onset diabetes of the young type 3 (MODY3) and also can result in the appearance of hepatic adenomas [taken from NCBI Entrez Gene (Gene ID: 6927)].

6927

HNF1A

HNF1 homeobox A

P20823

Hepatocyte nuclear factor 1-alpha

Alternate Names

albumin proximal factor; hepatic nuclear factor 1; hepatocyte nuclear factor 1-alpha; HNF1; HNF-1A; HNF-1-alpha; HNF4A; IDDM20; interferon production regulator factor; LFB1; liver-specific transcription factor LF-B1; MODY3; TCF1; TCF-1; Transcription factor 1; transcription factor 1, hepatic; truncated hepatocyte nuclear factor 1 alpha

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.