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Rabbit anti-PU.1 Recombinant Monoclonal Antibody [BLR179J]

Bethyl Laboratories®
Bethyl Laboratories®

Catalog #

Validated
Target:

PU.1

Reactivity:

Human

,

Mouse

Host:

Rabbit

Clonality:

Recombinant Monoclonal

Clone:

BLR179J

Format:

Whole IgG

Immunogen:

between 220 and 270 (C-term)

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Purified

100 µl (50+ tests)

10 µl (5+ tests)

Product Details

Specifications
Verified Reactivity

Mouse,

Human

Antigen Species

Human

Concentration
1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, BSA-Free


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Additional Product Information

PU.1 is an ETS-domain transcription factor that activates gene expression during myeloid and B-lymphoid cell development. PU.1 binds to a purine-rich sequence known as the PU-box found near the promoters of target genes and regulates their expression in coordination with other transcription factors and cofactors. [taken from NCBI Entrez Gene (Gene ID: 6688)].

6688

SPI1

Spi-1 proto-oncogene

P17947

Transcription factor PU.1

Alternate Names

31 kDa transforming protein; 31 kDa-transforming protein; hematopoietic transcription factor PU.1; OF; PU.1; SFPI1; SPI-1; SPI-A; spleen focus forming virus (SFFV) proviral integration oncogene spi1; transcription factor PU.1

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.