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Rabbit anti-Occludin Recombinant Monoclonal Antibody [BLR126H]

Bethyl Laboratories®
Bethyl Laboratories®

Catalog #

Validated
Target:

Occludin

Reactivity:

Human

Host:

Rabbit

Clonality:

Recombinant Monoclonal

Clone:

BLR126H

Format:

Whole IgG

Immunogen:

between 472 and 522 (C-term)

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Purified

100 µl (10 blots)

20 µl (2 blots)

100 µg (BSA-Free)

Product Details

Specifications
Verified Reactivity

Human

Antigen Species

Human

Concentration
100 µg/ml 1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide


Request Formulation Change

Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, BSA-Free


Request Formulation Change

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Additional Product Information

Occludin may play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier. It is able to induce adhesion when expressed in cells lacking tight junctions [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q16625].

100506658

OCLN

occludin

Q16625

Occludin

Alternate Names

BLCPMG; occludin; phosphatase 1, regulatory subunit 115; PPP1R115; PTORCH1; tight junction protein occludin

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.