/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-Arginase 1 Recombinant Monoclonal Antibody [BLR036F]
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Primary Antibodies  

Rabbit anti-Arginase 1 Recombinant Monoclonal Antibody [BLR036F]

Bethyl Laboratories®
Bethyl Laboratories®

Catalog #

Validated
Target:

Arginase 1

Reactivity:

Human

Applications:

ICC

,

IHC

,

IHC-IF

,

IP

,

WB

Host:

Rabbit

Clonality:

Recombinant Monoclonal

Clone:

BLR036F

Format:

Whole IgG

Immunogen:

residues 1-322 (FL)

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Purified

100 µl (50+ tests)

10 µl (5+ tests)

100 µg (BSA-Free)

Product Details

Specifications
Verified Reactivity

Human

Antigen Species

Human

Concentration
250 µg/ml 1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide


Request Formulation Change

Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, BSA-Free


Request Formulation Change

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Additional Product Information

Arginase1 is a key element of the urea cycle converting L-arginine to urea and L-ornithine, which is further metabolized into metabolites proline and polyamides that drive collagen synthesis and bioenergetic pathways critical for cell proliferation, respectively; the urea cycle takes place primarily in the liver and, to a lesser extent, in the kidneys. Arginase catalyzes the hydrolysis of arginine to ornithine and urea. At least two isoforms of mammalian arginase exist (types I and II) which differ in their tissue distribution, subcellular localization, immunologic crossreactivity and physiologic function. The type I isoform encoded by this gene, is a cytosolic enzyme and expressed predominantly in the liver as a component of the urea cycle. Inherited deficiency of this enzyme results in argininemia, an autosomal recessive disorder characterized by hyperammonemia.  [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P05089].

383

ARG1

arginase 1

P05089

Arginase-1

Alternate Names

arginase, liver; arginase-1; liver-type arginase; type I arginase

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.