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Primary Antibodies  

Rabbit anti-UBA6 Antibody

Bethyl Laboratories®
Bethyl Laboratories®

Catalog #

Validated
Target:

UBA6

Reactivity:

Human

Applications:

IP

,

WB

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

Between 1002 and 1052

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

Product Details

Specifications
Verified Reactivity

Human

Antigen Species

Human

Concentration
1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide


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Additional Product Information

Ubiquitin-like modifier-activating enzyme 6 (UBA6) activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding an ubiquitin-E1 thioester and free AMP. UBA6 is specific for ubiquitin and does not activate ubiquitin-like peptides. UBA6 differs from UBE1 in its specificity for substrate E2 charging. UBA6 does not charge cell cycle E2s, such as CDC34. UBE6 is essential for embryonic development and required for UBD/FAT10 conjugation [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/A0AVT1].

55236

UBA6

ubiquitin like modifier activating enzyme 6

A0AVT1

Ubiquitin-like modifier-activating enzyme 6

Alternate Names

E1-L2; monocyte protein 4; MOP-4; UBA6, ubiquitin-activating enzyme E1; UBE1L2; ubiquitin-activating enzyme 6; ubiquitin-activating enzyme E1-like 2; ubiquitin-activating enzyme E1-like protein 2; ubiquitin-like modifier-activating enzyme 6

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.