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Primary Antibodies  

Rabbit anti-c-Kit Antibody

Bethyl Laboratories®
Bethyl Laboratories®

Catalog #

Validated
Target:

c-Kit

Reactivity:

Human

Applications:

IHC

,

IP

,

WB

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

between 925 and 976

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

Product Details

Specifications
Verified Reactivity

Human

Antigen Species

Human

Concentration
1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide


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Additional Product Information

c-Kit is the receptor for stem cell factor (mast cell growth factor). It is a transmembrane receptor with tyrosine-protein kinase activity. Binding of the ligands leads to the autophosphorylation of the c-Kit receptor and its association with substrates such as phosphatidylinositol 3-kinase (Pi3K) [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/ P10721].

3815

KIT

KIT proto-oncogene receptor tyrosine kinase

P10721

Mast/stem cell growth factor receptor Kit

Alternate Names

CD antigen CD117; CD117; C-Kit; c-Kit protooncogene; mast/stem cell growth factor receptor Kit; MASTC; p145 c-kit; PBT; piebald trait protein; proto-oncogene c-Kit; proto-oncogene tyrosine-protein kinase Kit; SCFR; soluble KIT variant 1; tyrosine-protein kinase Kit; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.