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Primary Antibodies  

Rabbit anti-p66alpha Antibody

Bethyl Laboratories®
Bethyl Laboratories®

Catalog #

Validated
Citations (1)
Target:

p66alpha

Reactivity:

Human

Applications:

IP

,

WB

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

between 225 and 275

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

Product Details

Specifications
Verified Reactivity

Human

Antigen Species

Human

Concentration
1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide


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Additional Product Information

p66alpha and p66beta are human orthologs of the xenopus p66 subunit of the Mi-2/NuRD complex. The Mi-2/NuRD complex is a multi-subunit complex that mediates gene repression via processes involving chromatin remodeling and histone deacetylation. p66 alpha and beta were identified in a two-hybrid screen using the methyl-CpG-binding domain protein, MBD2, as bait. It has been demonstrated that p66alpha and p66beta mediate the interaction between MBD2 and histones.

54815

GATAD2A

GATA zinc finger domain containing 2A

Q86YP4

Transcriptional repressor p66-alpha

Alternate Names

GATA zinc finger domain-containing protein 2A; Hp66alpha; p66alpha; transcriptional repressor p66-alpha

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.