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Primary Antibodies  

Rabbit anti-PHD2 Antibody

Bethyl Laboratories®
Bethyl Laboratories®

Catalog #

Validated
Citations (19)
Target:

PHD2

Reactivity:

Human

Applications:

IP

,

WB

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

between 1 and 50

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

Product Details

Specifications
Verified Reactivity

Human

Antigen Species

Human

Concentration
1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide


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Additional Product Information

PHD2 is one of 4 PHD (PHD1-4) enzymes that function as oxygen sensors and are responsible for the post-translational modification of HIF-1alpha, a component of a transcriptional complex involved in oxygen homeostasis. During normoxic levels, PHDs catalyze the hydroxylation of prolyl residues on HIF-1alpha and target it for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. The PHD isoforms appear to function in a non-redundant manner and may differ in their expression patterns and their catalytic selectivity.

54583

EGLN1

egl-9 family hypoxia inducible factor 1

Q9GZT9

Egl nine homolog 1

Alternate Names

C1orf12; ECYT3; egl nine homolog 1; egl nine-like protein 1; HALAH; HIFPH2; HIF-PH2; HIF-prolyl hydroxylase 2; HPH2; HPH-2; hypoxia-inducible factor prolyl hydroxylase 2; PHD2; prolyl hydroxylase domain-containing protein 2; SM20; SM-20; zinc finger MYND domain-containing protein 6; ZMYND6

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.