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Primary Antibodies  

Rabbit anti-PCNA Antibody

Bethyl Laboratories®
Bethyl Laboratories®

Catalog #

Validated
Citations (10)
Target:

PCNA

Reactivity:

Human

,

Mouse

Applications:

Flow Cyt

,

IHC

,

IP

,

WB

Platforms:

PhenoImager™ HT

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

between 75 and 125

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

Product Details

Specifications
Verified Reactivity

Human,

Mouse

Presumed Reactivity

Chicken,

Bovine,

Rat

Antigen Species

Human

Concentration
1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide


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Additional Product Information

PCNA (Proliferating Cell Nuclear Antigen) was first identified as a factor expressed in the nuclei of replicating cells. PCNA plays a role in both DNA synthesis and repair. PCNA functions as a processivity factor for DNA polymerase by acting as a DNA clamp that tethers the polymerase catalytic units to the DNA template. The structure of PCNA is that of a trimeric ring that encircles and slides along replicating DNA. PCNA interacts with and is regulated by several proteins involved in cell cycle control and check point processes.

5111

PCNA

proliferating cell nuclear antigen

P12004

Proliferating cell nuclear antigen

Alternate Names

ATLD2; cyclin; DNA polymerase delta auxiliary protein; PCNA; proliferating cell nuclear antigen

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.