Western Blot Troubleshooting Questionnaire

If you are experiencing difficulties with a Bethyl product, please complete the form as accurately as possible. The information is helpful to troubleshoot the differences between the observed results and the expected results. Once submitted a Technical Support representative will contact you within 1 business day. Please note that all comments and suggestions are taken seriously by Bethyl Laboratories.

Customer Information

First Name Required Please enter your first name

Last Name Required Please enter your last name

Email Required Privacy Policy Please enter a valid email address

Confirm Email Confirm email and email must match

Institution

Phone

Product Information

Catalog Number Required Please enter the catalog number

Lot Number Where do I find my lot number?

Description

Date Received Please enter a valid date

PO Number

How many tests have been performed

Prior Use Required Please select prior use

I have never used this product before, and I am having trouble with this product.

I have successfully used a prior lot of this product, but I am having trouble with the current lot.

Prior Lot Required Please enter the prior lot number

I have successfully used this product in the past, but I am having trouble with a new shipment of the same lot.

I have successfully used the product from this shipment, but now I am having trouble with it.

Please describe the specific problem you are experiencing Required Problem description is required

Sample

Cell Line or Tissue

Name of cell line or tissue

Species of cell line or tissue

Type of Sample

Whole cell lysate

Nuclear extract

Cytosolic prep

Chromatin extract

Membrane prep

Other

Please describe Required Required

Lysis Buffer

Components

Date Prepared

Protease Inhibitors

Included

No Protease inhibitors were used

Amount	Name

Phosphatase Inhibitors

Included

No Phosphatase inhibitors were used

Amount	Name

Lysate

Date Prepared

Storage Conditions

Electrophoresis

Gel

Brand (if pre-cast)

Percentage Acrylamide

Buffer System

Amount of protein loaded per lane (μg)

Transfer

Method

Submersion

Semi-dry

Quick Transfer

Membrane

Nitrocellulose

PVDF

Amount of time for transfer

Western Blotting Procedure

Blocking

Formulation of blocking buffer

Length of time in block

Primary Antibody

Dilution

Diluent (buffer)

Incubation Time

Incubation Temperature

Secondary Antibody

Source/Vendor/Brand

Host Species

Target Species

Conjugate Type

Dilution

Diluent (buffer)

Incubation Time

Date of last successful use

Detection Method

Chemiluminescence

Brand (Amersham, Pierce, etc.)

Type (Dura-West, ECL-Plus, etc.)

Exposure

Film

CCD Camera

Time(s) for exposure(s)

Fluorescence

Imaging System

Acquisition Time(s)

Colorimetric

Brand

Type

Date of last successful use

Controls

Positive Control

Negative Control

Replicates

Number of times experiment was performed

Were the results the same each time

Yes

Please describe the differences

Other Comments

Uploads

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