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Primary Antibodies  

Rabbit anti-RNF216 Antibody

Bethyl Laboratories®
Bethyl Laboratories®

Catalog #

Validated
Citations (3)
Target:

RNF216

Reactivity:

Human

,

Mouse

Applications:

IP

,

WB

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

Between 100 and 150

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

Product Details

Specifications
Verified Reactivity

Human,

Mouse

Antigen Species

Human

Concentration
1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide


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Additional Product Information

Ring finger protein 216 (RNF216) specifically colocalizes and interacts with the serine/threonine protein kinase, receptor-interacting protein (RIP). Zinc finger domains RNF216 are required for its interaction with RIP and for inhibition of TNF- and IL1-induced NF-kappa B activation pathways. RNF216 may also function as an E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes and transfers it to substrates [taken from NCBI Entrez Gene (Gene ID: 10084)].

54476

RNF216

ring finger protein 216

Q9NWF9

E3 ubiquitin-protein ligase RNF216

Alternate Names

CAHH; E3 ubiquitin-protein ligase RNF216; RING finger protein 216; RING-type E3 ubiquitin transferase RNF216; triad domain-containing protein 3; TRIAD3; U7I1; UBCE7IP1; ubiquitin-conjugating enzyme 7-interacting protein 1; ZIN; zinc finger protein inhibiting NF-kappa-B

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.