What protocol was used to perform the blot shown in the data sheet of the Bethyl antibodies?
If I don’t use the ReliaBLOT® kit, what blocking buffer should I use?
The recipe for ReliaBLOT® blocking buffer is proprietary. An acceptable substitute would be 5% milk (Carnation, non-fat, dry)/TBS/0.5% tween. A protocol for Western Blotting can be found in the Protocols Section of the Bethyl website.
Can you give me the exact sequence of the peptide used as the immunogen to generate the antibody?
The sequences of the immunogens are proprietary. All of the data sheets on our polyclonal antibodies provide a range of amino acids in the protein to which the peptide maps.
Why is my band/signal not migrating at the same size that you show in your data sheet?
Why do some proteins not migrate at the predicted molecular weight?