Bethyl's purpose is to improve lives by supporting scientific discovery. This drives our complete dedication to the highest standards of product and service quality.
Bethyl is unique in our end-to-end control of the entire antibody lifecycle. From our veterinary facilities to the development, production and validation labs, the Bethyl team focuses on delivering quality products. This attention to quality at every step and the integration across each stage of the process means scientists can rely on Bethyl antibodies. We also offer expert technical support to help ensure your success.
Recognizing that your research and time are valuable, we promise to stand behind our products. Should one of our antibodies fail to return satisfactory results when used in the species, application and under the same conditions stated on the product datasheet, Bethyl will offer a full product replacement, credit or refund at the investigator's discretion. Before providing product replacement, credit or refund, we ask the investigator to complete an application specific questionnaire and work with our Technical Support team to identify potential steps to optimize conditions.
Every Bethyl product ships with a 100% guarantee; choose Bethyl antibodies with confidence.
• Specific: Binding is restricted to the target
• Selective: Binding favors the target
• Reproducible: Performance is repeatable
Bethyl’s ApproachStep 1) Antigen Design
The validation of our antibodies begins with antigen design:
Western blot (WB) and Immunoprecipitation (IP)WB is used in conjunction with IP to evaluate specificity and selectivity:
1. Endogenous target protein is immunoprecipitated with two or more antibodies raised against distinct epitopes.
2. Immunoprecipitates are immunoblotted with each of the epitope-distinct antibodies.
Bethyl qualifies antibodies exhibiting:• Specific or selective recognition of the target protein
Immunohistochemistry (IHC)For the evaluation of antibodies in IHC, multiple antibodies generated against distinct epitopes are used to demonstrate concordance:
1. Two or more antibodies against distinct epitopes are used to stain a broad spectrum of FFPE tissues and cells.
2. When selectivity is established, Bethyl scientists find the optimal conditions and dilutions for reproducible immunostaining.
3. Alternately if more than one antibody exhibiting utility in IHC is not available, the scientific team confirms that the observed staining pattern is in agreement with published studies and curated data.
Bethyl qualifies antibodies for the application of IHC exhibiting:
Bethyl's validation process is continuous and lot-to-lot consistency is important. New lots are routinely evaluated in side-by-side comparisons with previous lots to assess lot-to-lot consistency. Each new lot must perform similarly to the previous lots in order to be released to market.
Interested in learning more about Bethyl’s validation process? Read the Application Note published in BioTechniques by clicking here.