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TIM-3 for a Change

Contributed by Kimberly Holloway, Ph.D.

The Resistance

Immune-checkpoint inhibitors is a popular topic of current discussion within the realm of tumor therapeutics.  Most notably cytotoxic T lymphocyte antigen 4 (CTLA-4) and programmed death receptor 1 (PD-1) have garnered the most attention.   The first immune checkpoint inhibitor associated with overall survival within a phase 3 metastatic melanoma study, Ipilimumab, is a fully humanized antibody against cytotoxic T-lymphocyte–associate-d antigen 4 (CTLA-4) antibody. 1,2 Blockade of PD-1 has achieved revolutionary clinical impact in many solid cancers; however, there has been certain roadblocks which have prevented full response to anti-PD-1 therapy.3–5 A significant percentage of cancer patients fail to respond to these therapies due to compensatory immune inhibitory pathways. 6

TIM-3 is now

In 2016, Koyama and colleagues compared the immune cells present in effusion samples collected from patients whom developed resistance to anti-PD-1 therapy with that of immune cells from five patients with non-small-cell lung cancer who had not received anti-PD-1 treatment.7  Higher levels of TIM-3 were detected in T cells from the PD-1 resistant patients compared with the other patients.7  T-cell immunoglobin mucin-3, TIM-3, is highly expressed on tumor infiltrating dendritic cells and actively competes with nucleic acids released from dying tumor which effectively inhibits stimulation of the innate immune response by nucleic acids. 6, 8 This weakening of innate immunity by TIM-3 exposes a major vulnerability in which tumor cells are now able to bypass pro-inflammatory cytokines.  In addition, Tim-3 inhibits immune responses and promotes tolerance by regulating multiple targets including CD4+ T, CD8+ T, T-regulatory (Tregs), Forkhead Box P3 (FoxP3), Type 1 regulatory T (Tr1), Natural killer (NK), dendritic (DCs), and myeloid-derived suppressor (MDSCs) cells.9 Such findings suggest the therapeutic targeting of TIM-3 within the context of cancer.  However, due to its extensive role in immune response, TIM-3 mAbs can also influence other pathophysiological mechanisms such as the severity of experimental autoimmune encephalomyelitis (EAE).10 In addition, administration of TIM-3 mAbs has found to not inhibit tumor growth on its own.11 Therefore, careful thought on how and when to use TIM-3 mAbs should be considered.


A Compromise

In 2010, it was shown that combination of TIM-3 and PD-1 mAbs had a much greater antitumor effect than administration of TIM-3 or PD-1 mAbs alone.11  Therefore, a two-tier therapeutic model in which CTLA-4 and PD-1 represent the first tier of co-inhibitory receptors that are primarily responsible for maintaining self-tolerance and the second tier which includes TIM-3 as a co-inhibitory molecules that regulates immune responses at sites of tissue inflammation has been introduced as an alternative resolution to resistance.9 As expected, the effect of PD-1 blockade is proportionally larger than that of Tim-3 blockade alone. However, Tim-3 blockades preferentially affect tumor tissue Treg and IL-10-producing Tr1 cells while additionally affecting dendritic cell phenotype and dampening MDSCs. Thus, there has been a consensus that different checkpoint receptor blockades can be combined to achieve distinct effects on the immune response.9 


Detection of Human TIM3 in FFPE tonsil by IHC.

Detection of Human TIM3 in FFPE tonsil by IHC. Antibody: Rabbit anti-TIM3 recombinant monoclonal [BLR033F] (A700-033). Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: DAB.


Detection of human TIM3 In FFPE RPMI-8226 cells by ICC.

Detection of human TIM3 In FFPE RPMI-8226 cells by ICC. Antibody: Rabbit anti-TIM3 recombinant monoclonal [BLR033F] (A700-033) Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: DAB.


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2. Robert C, Thomas L, Bondarenko I, O’Day S, Weber J, Garbe C, Lebbe C, Baurain J-F, Testori A, Grob J-J, et al. Ipilimumab plus dacarbazine for previously untreated metastatic melanoma. The New England Journal of Medicine. 2011;364(26):2517–2526. doi:10.1056/NEJMoa1104621

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