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Lysis buffers: RIPA vs. NETNArticleCell lysis is a critical step for many different types of molecular and cellular assays. These assays rely on solubilizing cellular material like proteins or DNA for analysis, and the quality of the assay performed is based on the efficiency of the extraction of these materials. With multiple lysis buffers to choose from, how do you know which is right for you? Here, we focus on two frequently used lysis buffers RIPA (RadioImmunoPrecipitation Assay) and NETN.
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Retrieval buffers: Citrate vs. Tris-EDTAArticleAntigen retrieval is an important step in immunohistochemistry performed on formalin-fixed, paraffin-embedded (FFPE) tissues. When tissue is fixed, the antigens get cross-linked to preserve the cells and tissue in as close to a native state as possible. Fixation increases the longevity of cells and tissue but are chemically modified in the process. These chemical modifications that stabilize and strengthen the tissue can mask antigens and result in poor antibody detection. To combat this, antigen retrieval is performed in one of two main ways: Proteolytic-Induced Epitope Retrieval (PIER) or Heat-Induced Epitope Retrieval (HIER). In this feature we are going to discuss two different buffers used in HIER: citrate and Tris-EDTA.
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Human Umbilical Cord Derived Mesenchymal Stem Cell Sheets for Clinical UseAmber Miller, Ph.D.ArticleWith the incredible therapeutic potential of human umbilical derived mesenchymal stem cell sheets, manufacturing, preservation, and quality standards needed to be developed to ensure patient safety. Researchers developed culture and preservation methods that were able to be scaled up into a two-tiered cell bank system that maintained quality, integrity, and functionality of the mesenchymal stem cells.
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Targeting cancer stem cells with dendritic cell vaccines in melanomaAmber Miller, Ph.D.ArticleDendritic cell vaccines targeting aldehyde dehydrogenases via peptides to ALDH1A1 and ALDH1A3 were able to reduce cancer stem cell numbers, decrease tumor growth, and increase T-cell infiltration and cytotoxic activity. This activity was enhanced with the addition of PD-L1 blockade providing additional therapeutic strategies to improve the efficacy of immune checkpoint blockade.
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How to Interpret Flow Cytometry DataArticleThe experiment is complete. The samples have been run through the flow cytometry machine. Now it’s time for the fun part, examining the results of your experiment!
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