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Evolution and Future of Multiplex Immunohistochemistry using Tyramide Signal Amplification

Evolution and Future of Multiplex Immunohistochemistry using Tyramide Signal Amplification

Contributed by Jane Naberhuis, Ph.D.

 

Much of the early utility of IHC was confined to the basic science lab, but the developments described here have helped shift fluorescent mIHC with TSA into a powerful tool for translational research. Moving forward, additional technical advancements and more widespread affordability and availability of imaging technology will likely see mIHC transition to an integrated, workhorse clinical tool. Clinical use will require highly reproducible, quantifiable results, as well as adherence to regulatory requirements.

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