DHX16 is a member of the DEAH-box family of proteins that possesses several conserved motifs which include the highly conserved DEAH (Asp-Glu-Ala-His) amino acid sequence motif. The major activity of DEAH-box proteins is to function as ATP-dependent RNA helicases. RNA helicases play an important role in all aspects of RNA metabolism and function which include pre-mRNA splicing, RNA synthesis, RNA degradation, RNA export, RNA translation, RNA secondary structure formation, ribosome biogenesis, and the assembly of RNP complexes. DH16 was originally identified as DBP2 and discovered to be a functional homolog of the yeast RNA helicase and ATPase, Cdc28/Prp68. DHX16 appears to play a key role in nonsense-mediated mRNA decay (NMD) as well as rRNA processing.