Overview of Cell Biology

Jump to Antibodies

The study of cell biology deals with the molecular biology and biochemistry of cell function, including how cells process signals from their external environment such as nutrients, growth factors, and cell-cell contact. It also deals with the structure and function of organelles, membranes, receptors, and macromolecular turnover. Signal transduction initiates the biochemical processes of DNA, RNA, and protein synthesis, gene expression, cell division, and cell death mechanisms, and regulates cell metabolism and energy production. Environmental sensing and intracellular signaling also regulate molecular reorganization, shape, and motility, as well as translocation of molecules within the cell to and from the surface and external milieu, e.g. phagocytosis, endocytosis, and secretion.

Bethyl’s cell biology portfolio consists of over 9,000 antibodies. Our antibodies have been manufactured on-site by our own scientists. Bethyl’s rigorous validation process ensures that the antibody will work in the applications outlined on the datasheet. These applications include: Western blot, immunohistochemistry, immunoprecipitation, immunocytochemistry, flow cytometry, proximity ligation assay, and ChIP. A Bethyl antibody is first validated for specificity in immunoprecipitation and/or western blot and if it passes our team will validate the antibody for additional applications. Our products are backed with a 100% guarantee to work in your assay, when used in under the same conditions outlined on the product datasheet, which provides confident, reliable results.

Bethyl sells a wide variety of antibodies for cell biology research. Recent publications using our antibodies for studying cell biology include:

  • A new platform designed to identify proteins associated with sarcomas1
  • Identifying a novel protein involved in DNA repair pathways at telomeres that contribute to aging and cancer2
  • Exploring how the protein translation cellular machinery governs a set of mRNAs associated with cell cycle control and apoptosis3
  • Examining how a series of proteins control the stability of mRNAs, which in turn regulates cellular processes such as the inflammasome4
  • isomerization of a known DNA damage response protein impacts its role with mitochondria outside the nucleus5

 

Detection of human KIF14 in formaldehyde-fixed asynchronous HeLa cells by ICC-IFF

: Detection of human KIF14 (orange) in formaldehyde-fixed asynchronous HeLa cells by ICC-IF. Antibody: Rabbit anti-KIF14 (A300-912A). Secondary: DyLight® 550 conjugated goat anti-rabbit (A120-101D3). Counterstain: Phalloidin Alexa Fluor® 488-conjugated (green) and DAPI (blue).

Detection of human and mouse CSTF50 by WB of HeLa, 293T, and NIH3T3 lysate.

Detection of human and mouse CSTF50 by WB of HeLa, 293T, and NIH3T3 lysate. Antibody: Rabbit anti-CSTF50 (A301-250A). Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P).

Detection of human HIF2-alpha in formaldehyde-fixed asynchronous HeLa cells by ICC-IF.

Detection of human HIF2-alpha (red) in formaldehyde-fixed, CoCl2 treated, HeLa cells by ICC-IF. Antibody: Rabbit anti-HIF2-alpha (A700-003).  Secondary: DyLight® 594 conjugated goat anti-rabbit (A120-201D4). Counterstain: Phalloidin Alexa Fluor® 488-conjugated (green) and DAPI (blue).

 

Below is the entire list of targets involved in cell biology research. Can’t find what you are looking for? Bethyl offers a custom antibody service.

 

References

1. Sundararaman B, Zhan L, Blue SM, Stanton R, Elkins K, Olson S, Wei X, Van Nostrand EL, Pratt GA, Huelga SC, et al. 2016. Resources for the Comprehensive Discovery of Functional RNA Elements. Mol Cell. Mar 17; 61(6): 903-913. [330 Bethyl antibodies were used; please refer to the article for the complete list Sundararaman, B. et al. (2016). Mol Cell. Mar 17; 61(6): 903-13. [330 Bethyl antibodies were used; please refer to the article for the complete list]

2. Boersma V, Boersma V, Moatti N, Segura-Bayona S, Peuscher MH, van der Torre J, Wevers BA, Orthwein A, Durocher D, Jacobs JJL. 2015. MAD2L2 controls DNA repair at telomeres and DNA breaks by inhibiting 5' end resection. Nature May 28;521(7553):537-540. [Bethyl antibody used: Phopsho RPA32 (S4/S8) Antibody (A300-245A)]

3. Lee AS, Kranzusch PJ, Cate JH. 2015. eIF3 targets cell-proliferation messenger RNAs for translational activation or repression. Nature Jun4;522(7554):111-114. [Bethyl antibodies used: GAPDH Antibody (A300-640A), eIF3G/EIF3S4 Antibody (A301-757A), eIF3B/EIF3S9 Antibody (A301-761A), eIF4G1/eIF4GI Antibody (A301-775A), c-Jun Antibody (A302-959A), eIF3E Antibody (A302-985A). Please note that some of these antibodies were used in CLIP. Bethyl has not validated it for use in this application at this time and cannot guarantee the same results as published.]

4. Hu G, McQuiston T, Bernard A, Park YD, Qiu J, Vural A, Zhang N, Waterman SR, Blewett NH, Myers TG, et al. 2015. A conserved mechanism of TOR-dependent RCK-mediated mRNA degradation regulates autophagy. Nat Cell Biol. July:17(7):930-942. [Bethyl antibody used: DDX6 Antibody (A300-461A)]

5. Hilton BA, Li Z, Musich PR, Wang H, Cartwright BM, Serrano M, Zhou XZ, Lu KP, Zou Y. 2015 ATR Plays a Direct Antiapoptotic Role at Mitochondria, which Is Regulated by Prolyl Isomerase Pin1. Mol Cell. Oct 1;60(1):35-46. [Bethyl antibody used: ATR Antibody (A300-138A)]