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Primary Antibodies  

Rabbit anti-MCM10 Antibody

Bethyl Laboratories®
Bethyl Laboratories®

Catalog #

Validated
Citations (8)
Target:

MCM10

Reactivity:

Human

Applications:

WB

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

between 600 and 650

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

Product Details

Specifications
Verified Reactivity

Human

Antigen Species

Human

Concentration
1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide


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Additional Product Information

The MCM (Mini-Chromosome Maintenance) complex is a key component of the pre-replication complex involved in replication licensing which restricts DNA replication to only once per cell cycle. The MCM complex is a heterohexamer of MCM2, MCM3, MCM4, MCM5, MCM6, and MCM7. MCM10 (Mini-Chromosome Maintenance 10) is not a member of the canonical six that form the MCM complex. MCM10 appears to interact with all members of the MCM complex and functions as a factor that recruits the MCM complex to the pre-replication complex (pre-RC) on DNA.

55388

MCM10

minichromosome maintenance 10 replication initiation factor

Q7L590

Protein MCM10 homolog

Alternate Names

CNA43; DNA43; homolog of yeast MCM10; hsMCM10; MCM10 minichromosome maintenance deficient 10; minichromosome maintenance complex component 10; PRO2249; protein MCM10 homolog

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.