/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-6-His Tag Antibody
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Primary Antibodies  

Rabbit anti-6-His Tag Antibody

Bethyl Laboratories®
Bethyl Laboratories®

Catalog #

Citations (19)
Citations (6)
Citations (2)
Target:

6-His Tag

Applications:

ELISA

,

ICC

,

IF

,

IP

,

WB

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Isotype:

IgG

Conjugate:

Biotin

,

FITC

,

HRP

,

Unconjugated

Purity:

Antigen Affinity Purified

Unconjugated (0.1 mg)

Biotin (0.1 mg)

FITC (0.1 mg)

HRP (0.1 mg)

Product Details

Specifications
Concentration
1 mg/ml
Fluorophore/Protein
3.8
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Phosphate Buffered Saline (PBS) containing 0.09% Sodium Azide


Request Formulation Change

Phosphate Buffered Saline (PBS) containing 0.2% BSA and 0.05% Pro-Clean 400


Request Formulation Change

Phosphate Buffered Saline (PBS) containing 0.2% BSA and 0.09% Sodium Azide


Request Formulation Change

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Additional Product Information

The 6-His tag is a commonly used epitope tag that can be added to a recombinantly expressed protein for the purposes of protein purification or detection of protein. Typically utilizes metal affinity chromatography for purification and elutes well imidazole.

Applications

Not all listed applications have been specifically tested by our laboratory. All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer. All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).