Two-color Western Blot Detection of Actin and hnRNP in HeLa Whole Cell Lysate. Primary antibodies: cocktail of rabbit anti-Actin A300-491A (A300-491A-5) and mouse anti-hnRNP A500-011A (A500-011A-1) at 1 µg/ml each. Secondary antibodies: cocktail of Dylight®800-conjugated goat anti-rabbit A120-201D8 (A120-201D8-1) (green) and Dylight® 488-conjugated goat anti-mouse A90-516D2 (A90-516D2-5) (blue) at 0.5 µg/ml each. Acquisition: Syngene G:Box, 17 seconds (green) and 34 seconds (blue).
Detection of Human p53 by Immunofluorescence. Sample: FFPE section of human stomach carcinoma. Primary Antibody: Mouse anti-p53 (clone DO-1) used at a dilution of 1:100. Secondary Antibody: Green-fluorescent Goat anti-Mouse IgG-heavy and light chain cross-adsorbed Antibody DyLight® 488 Conjugated (A90-516D2 Lot 5) used at a dilution of 1:50 (10µg/ml). Counterstain: DAPI (blue)