Two-color Western Blot Detection of GAPDH and hnRNP in HeLa Whole Cell Lysate. Primary antibodies: cocktail of goat anti-GAPDH A303-878A (A303-878A-1) and mouse anti-hnRNP A500-011A (A500-011A-1) at 1 µg/ml each. Secondary antibodies: cocktail of Dylight® 680-conjugated rabbit anti-goat A50-200D6 (A50-200D6-1) (red) and Dylight® 488-conjugated rabbit anti-mouse A90-317D2 (A90-317D2-3) (blue) at 0.5 µg/ml each. Acquisition: Syngene G:Box, 39 seconds (red) and 60 seconds (blue).
Detection of Human p53 by Immunofluorescence. Sample: FFPE section of human breast carcinoma. Primary Antibody: Mouse anti-p53 (clone DO-1) used at a dilution of 1:100. Secondary Antibody: Green-fluorescent Rabbit anti-Mouse IgG-heavy and light chain cross-adsorbed Antibody DyLight® 488 Conjugated (A90-317D2 Lot 6) used at a dilution of 1:100 (5µg/ml). Counterstain: DAPI (blue)