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Primary Antibodies  

Rabbit anti-PAN3 Antibody

Bethyl Laboratories®
Bethyl Laboratories®

Catalog #

Validated
Citations (1)
Target:

PAN3

Reactivity:

Human

Applications:

IP

,

WB

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

Between 175 and 225

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

Product Details

Specifications
Verified Reactivity

Human

Antigen Species

Human

Concentration
1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide


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Additional Product Information

PAB-dependent poly(A)-specific ribonuclease subunit PAN3 is a regulatory subunit of the poly(A)-nuclease (PAN) deadenylation complex, one of two cytoplasmic mRNA deadenylases involved in general and miRNA-mediated mRNA turnover. PAN specifically shortens poly(A) tails of RNA when the poly(A) stretch is bound by poly(A)-binding protein (PABP), which is followed by rapid degradation of the shortened mRNA tails by the CCR4-NOT complex. Deadenylated mRNAs are then degraded by two alternative mechanisms, namely exosome-mediated 3'-5' exonucleolytic degradation, or deadenlyation-dependent mRNA decaping and subsequent 5'-3' exonucleolytic degradation by XRN1. PAN3 acts as a positive regulator for PAN activity, recruiting the catalytic subunit PAN2 to mRNA via its interaction with PABP and to miRNA targets via its interaction with GW182 family proteins [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q58A45].

255967

PAN3

poly(A) specific ribonuclease subunit PAN3

Q58A45

PAB-dependent poly(A)-specific ribonuclease subunit PAN3

Alternate Names

PAB1P-dependent poly(A)-nuclease; PAB1P-dependent poly(A)-specific ribonuclease; PAB-dependent poly(A)-specific ribonuclease subunit 3; PAB-dependent poly(A)-specific ribonuclease subunit PAN3; PABP1-dependent poly A-specific ribonuclease subunit PAN3; PABP-dependent poly(A) nuclease 3; PAN deadenylation complex subunit 3; PAN2-PAN3 deadenylation complex subunit PAN3; PAN3 poly(A) specific ribonuclease subunit homolog; poly(A)-nuclease deadenylation complex subunit 3

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.