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Primary Antibodies  

Rabbit anti-PRPF8 Antibody

Bethyl Laboratories®
Bethyl Laboratories®

Catalog #

Validated
Citations (4)
Target:

PRPF8

Reactivity:

Human

,

Mouse

Applications:

IHC

,

IP

,

WB

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

Between 1825 and 1875

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

Product Details

Specifications
Verified Reactivity

Human,

Mouse

Antigen Species

Human

Concentration
1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide


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Additional Product Information

Pre-mRNA-processing-splicing factor 8 (PRPF8) is a central component of the spliceosome, which may play a role in aligning the pre-mRNA 5'- and 3'-exons for ligation. PRPF8 interacts with U5 snRNA, and with pre-mRNA 5'-splice sites in B spliceosomes and 3'-splice sites in C spliceosomes [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q8TCS8].

10594

PRPF8

pre-mRNA processing factor 8

Q6P2Q9

Pre-mRNA-processing-splicing factor 8

Alternate Names

220 kDa U5 snRNP-specific protein; apoptosis-regulated protein 1; apoptosis-regulated protein 2; HPRP8; p220; precursor mRNA processing protein; pre-mRNA-processing-splicing factor 8; PRP8; PRP8 homolog; PRP8 pre-mRNA processing factor 8 homolog; PRPC8; RP13; SNRNP220; splicing factor Prp8; U5 snRNP-specific protein (220 kD), ortholog of S. cerevisiae Prp8p

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.