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Primary Antibodies  

Rabbit anti-NF45 Antibody

Bethyl Laboratories®
Bethyl Laboratories®

Catalog #

Validated
Citations (8)
Target:

NF45

Reactivity:

Human

,

Mouse

Applications:

IHC

,

IP

,

WB

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

between 340 and 390

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

Product Details

Specifications
Verified Reactivity

Mouse,

Human

Presumed Reactivity

X_tropicalis,

Rat

Antigen Species

Human

Concentration
1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide


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Additional Product Information

The 45kDa nuclear factor of activated T-cells (NF45) functions as part of a heterodimeric complex with NF90 to form the transcription factor, NFAT (nuclear factor of activated T-cells). The NF45/NF90 complex binds the interleukin-2 promoter and regulates transcription of the IL2 gene during T-cell activation. It has also been shown that the NF45/NF90 complex can function as a negative regulator of miRNA biogenesis.

3608

ILF2

interleukin enhancer binding factor 2

Q12905

Interleukin enhancer-binding factor 2

Alternate Names

interleukin enhancer binding factor 2, 45kD; interleukin enhancer binding factor 2, 45kDa; interleukin enhancer-binding factor 2; NF45; Nuclear factor of activated T-cells 45 kDa; nuclear factor of activated T-cells, 45-kDa; PRO3063

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.