/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-Cortactin Antibody
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Primary Antibodies  

Rabbit anti-Cortactin Antibody

Bethyl Laboratories®
Bethyl Laboratories®

Catalog #

Validated
Target:

Cortactin

Reactivity:

Human

Applications:

IP

,

WB

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

between 250 and 300

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (200 µg/ml)

10 µl (200 µg/ml)

Product Details

Specifications
Verified Reactivity

Human

Presumed Reactivity

Chicken

Antigen Species

Human

Concentration
200 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide


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Additional Product Information

Cortactin has two roles: (1) regulating the interactions between components of adherens-type junctions and (2) organizing the cytoskeleton and cell adhesion structures of epithelia and carcinoma cells. The aberrant regulation of cortactin contributes to tumor cell invasion and metastasis [taken from NCBI Entrez Gene (GeneID: 2017)].

2017

CTTN

cortactin

Q14247

Src substrate cortactin

Alternate Names

1110020L01Rik; amplaxin; EMS1; ems1 sequence (mammary tumor and squamous cell carcinoma-associated (p80/85 src substrate)); epididymis secretory sperm binding protein; oncogene EMS1; src substrate cortactin

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.