Detection of Human MYT1 by Western Blot and Immunoprecipitation.Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-MYT1 antibody A302-424A used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).
Myt1 kinase, PKmyt1 kinase, Membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase, protein kinase, membrane associated tyrosine/threonine 1, membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase
Antigen Affinity Purified
MYT1 (membrane associated tyrosine/threonine protein kinase 1) is is a member of the serine/threonine protein kinase family. This kinase preferentially phosphorylates and inactivates cell division cycle 2 protein (CDC2), and thus negatively regulates cell cycle G2/M transition. MYT is associated with the membrane throughout the cell cycle, and its activity is highly regulated during the cell cycle. Protein kinases AKT1/PKB and PLK (Polo-like kinase) have been shown to phosphorylate and regulate the activity of MYT1 [taken from NCBI Entrez Gene (GeneID:9088)].