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Rabbit anti-MEKK1 Antibody

Bethyl Laboratories®
Bethyl Laboratories®

Catalog #

Validated
Target:

MEKK1

Reactivity:

Human

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

between 50 and 100

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

Product Details

Specifications
Verified Reactivity

Human

Presumed Reactivity

Mouse,

Rat

Antigen Species

Human

Concentration
1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide


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Additional Product Information

MEK (MAPK/ERK kinase) kinase 1 (MEKK1) is encoded by the MAP3K1 (mitogen-activated protein kinase kinase kinase 1) gene. MEKK1 is a component of a protein kinase signal transduction cascade that activates the ERK and JNK kinase pathways by phosphorylation of MEK1 (MAP2K1) and MEK4 (MAP2K4). MEKK1 activates CHUK and IKBKB, the central protein kinases of the NF-kappa-B pathway [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q13233].

4214

MAP3K1

mitogen-activated protein kinase kinase kinase 1

Q13233

Mitogen-activated protein kinase kinase kinase 1

Alternate Names

MAP/ERK kinase kinase 1; MAPK/ERK kinase kinase 1; MAPKKK1; MEK kinase 1; MEKK; MEKK 1; MEKK1; mitogen-activated protein kinase kinase kinase 1; mitogen-activated protein kinase kinase kinase 1, E3 ubiquitin protein ligase; SRXY6

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.