Detection of Human Citron by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-Citron antibody A302-303A used for WB at 0.1 µg/ml (A) and 1 µg/ml (B) and used for IP at 10 µg/mg lysate. Citron was also immunoprecipitated by rabbit anti-Citron antibody BL5459, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).
Citron is a dual specificity serine/threonine protein kinase and a putative RHO/RAC effector that binds to the GTP-bound forms of RHO and RAC1. It is required for KIF14 localization to the central spindle and midbody and may play a role in cytokinesis. Citron also plays an important role in the regulation of cytokinesis and the development of the central nervous system [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/O14578]