Detection of Human and Mouse NARG1 by Western Blot (h&m) and Immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 µg) and mouse NIH3T3 (M; 50µg) cells.Antibodies: Affinity purified rabbit anti-NARG1 antibody A302-147A used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. NARG1 was also immunoprecipitated, albeit poorly, by rabbit anti-NARG1 antibody BL8491, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 10 seconds (A and B).
Detection of Human NARG1 by Immunofluorescence and Immunohistochemistry. Sample: FFPE sections of human testicular seminoma (left) and colon carcinoma (right). Antibody: Affinity purified rabbit anti-NARG1 (Cat. No. A302-147A Lot1) used at a dilution of 1:80 (2.5 µg/ml) and 1:200 (1µg/ml). Detection: Red-fluorescent Goat anti-Rabbit IgG-heavy and light chain, cross-adsorbed Antibody DyLight® 594 Conjugated (Cat. No. A120-601D4) used at a dilution of 1:100 and DAB.
Tbdn100, NAA15, GA19, NATH, Ga19, TBDN100, N-alpha-acetyltransferase 15, NatA auxiliary subunit, Gastric cancer antigen Ga19, N-terminal acetyltransferase, NMDA receptor-regulated protein 1, Protein tubedown-1, Nalpha)-acetyltransferase 15, Na
Antigen Affinity Purified
NARG1 (NMDA receptor-regulated 1) is also known as NATH (N-acetyltransferase). The NARG1 gene, along with NARG2 and NARG3, was originally identified as a gene regulated by NMDA receptors in developing neurons. The NARG1 gene was found to be a homolog of a yeast N-terminal acetyltransferase that functions in control of the G(0) phase of the cell cycle. NARG1 was also identified as NATH in experiments set out to identify genes differentially expressed in papillary thyroid carcinoma (PTC). The NATH protein has been shown to form a complex with human ARD1 where they function as an acetyltransferase and interact with ribosomal subunits. The ARD1/NATH complex plays an important role in cell survival.