/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-U2AF35 Antibody
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Primary Antibodies  

Rabbit anti-U2AF35 Antibody

Bethyl Laboratories®
Bethyl Laboratories®

Catalog #

Validated
Citations (3)
Target:

U2AF35

Reactivity:

Human

,

Mouse

Applications:

IP

,

WB

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

between 190 and 240

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (200 µg/ml)

10 µl (200 µg/ml)

Product Details

Specifications
Verified Reactivity

Human,

Mouse

Presumed Reactivity

Bovine

Antigen Species

Human

Concentration
200 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide


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Additional Product Information

U2AF35 heterodimerizes with U2AF65 to form the U2 snRNP auxiliary factor which is part of the splicesome. U2AF35 is required for splicing of AG-dependent pre-mRNAs and functions in recognizing the conserved 3’ splice site dinucleotide AG. U2AF35 contains an atypical RNA recognition motif (RRM), two C3H1-type zinc fingers, and a C-terminal arginine/serine (RS)-rich domain interrupted by glycines.

7307

U2AF1

U2 small nuclear RNA auxiliary factor 1

Q01081

Splicing factor U2AF 35 kDa subunit

Alternate Names

FP793; RN; RNU2AF1; splicing factor U2AF 35 kDa subunit; splicing factor U2AF 35kDa subunit; U2 auxiliary factor 35 kDa subunit; U2 small nuclear ribonucleoprotein auxillary factor, 35-KD subunit; U2 small nuclear RNA auxiliary factor 1; U2 small nuclear RNA auxillary factor 1; U2 snRNP auxiliary factor small subunit; U2(RNU2) small nuclear RNA auxiliary factor binding protein; U2AF35; U2AFBP

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.