/ ANTIBODIES/ANTISERA / MAPK Pathway Antibodies / Rabbit anti-ERK1 Antibody
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MAPK Pathway Antibodies  

Rabbit anti-ERK1 Antibody

Bethyl Laboratories®
Bethyl Laboratories®

Catalog #

Validated
Citations (3)
Target:

ERK1

Reactivity:

Human

Applications:

IHC

,

IP

,

WB

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

between 329 and 379

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (200 µg/ml)

10 µl (200 µg/ml)

Product Details

Specifications
Verified Reactivity

Human

Antigen Species

Human

Concentration
200 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide


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Additional Product Information

Extracellular signal-regulated kinase 1 (ERK1/p44), also known as mitogen-activated protein kinase 3 (MAPK3), and extracellular signal-regulated kinase 2 (ERK2/p42), also known as mitogen-activated protein kinase 1 (MAPK1), are part of a signal transduction pathway that begins with the small GTPase Ras and continues through a cascade of protein kinases that include Raf and MEK. Activation of this signaling pathway influences many cellular processes such as proliferation, differentiation and survival. ERK1 is very similar in sequence to ERK2/p42 and the activities of both ERK1 and ERK2 are usually considered together as total ERK activity. More recent evidence has shown that ERK1 and ERK2 are not functionally redundant and may have very different and specific roles.

5595

MAPK3

mitogen-activated protein kinase 3

P27361

Mitogen-activated protein kinase 3

Alternate Names

ERK1; ERK-1; ERT2; extracellular signal-regulated kinase 1; extracellular signal-related kinase 1; HS44KDAP; HUMKER1A; insulin-stimulated MAP2 kinase; MAP kinase 3; MAP kinase isoform p44; MAPK 1; MAPK 3; microtubule-associated protein 2 kinase; mitogen-activated protein kinase 3; P44ERK1; p44-ERK1; P44MAPK; p44-MAPK; PRKM3

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.