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Primary Antibodies  

Rabbit anti-JARID1B Antibody

Bethyl Laboratories®
Bethyl Laboratories®

Catalog #

Validated
Citations (17)
Target:

JARID1B

Reactivity:

Human

Applications:

IHC

,

IP

,

WB

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

between 1494 and 1544

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

Product Details

Specifications
Verified Reactivity

Human

Antigen Species

Human

Concentration
1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide


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Additional Product Information

JARID1B (Jumonji AT-rich interactive domain 1B) is a member of the JARID 1 family of proteins that catalyze the demethylation of histones on lysine. The Jarid 1 family members include JARID1A, JARID1B, JARID1C and JARID1D. The members of this family possess a Jumonji C (JmjC) and Jumonji N (JmjN) domain as well as one ARID (AT-rich interactive domain) and three PHD-type zinc fingers. As histone demethylases, JARID 1 proteins function as transcriptional repressors. JARID1B expression is largely restricted to testis and may play a critical role in tumorigenesis as it has been found to be upregulated in prostate and breast cancer.

10765

KDM5B

lysine demethylase 5B

Q9UGL1

Lysine-specific demethylase 5B

Alternate Names

cancer/testis antigen 31; CT31; histone demethylase JARID1B; JARID1B; jumonji, AT rich interactive domain 1B; jumonji/ARID domain-containing protein 1B; lysine (K)-specific demethylase 5B; lysine-specific demethylase 5B; MRT65; PLU1; PLU-1; PPP1R98; protein phosphatase 1, regulatory subunit 98; PUT1; putative DNA/chromatin binding motif; RBBP2H1A; RBP2-H1; retinoblastoma-binding protein 2 homolog 1; retinoblastoma-binding protein 2, homolog 1A

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.