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Primary Antibodies  

Rabbit anti-SMG1 Antibody

Bethyl Laboratories®
Bethyl Laboratories®

Catalog #

Validated
Citations (2)
Target:

SMG1

Reactivity:

Human

Applications:

WB

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

between 1250 and 1300

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

Product Details

Specifications
Verified Reactivity

Human

Presumed Reactivity

Mouse

Antigen Species

Human

Concentration
1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide


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Additional Product Information

SMG1 is a PIKK (phosphoinositide 3-kinase related kinases) protein kinase that plays a key role in RNA surveillance via nonsense-mediated mRNA decay (NMD). SMG1 phosphorylates Upf1, a factor that possesses RNA helicase activity and is also central to NMD. SMG1 and UPF-1 have been shown to exist in a complex with the translation termination release factors eRF3, and eRF1. This complex has been termed SURF and associates with the exon junction complex (EJC) to function in the recognition of premature termination codons in aberrant RNAs to trigger NMD.

23049

SMG1

SMG1, nonsense mediated mRNA decay associated PI3K related kinase

Q96Q15

Serine/threonine-protein kinase SMG1

Alternate Names

61E3.4; ATX; hSMG-1; lambda/iota protein kinase C-interacting protein; lambda-interacting protein; LIP; PI-3-kinase-related kinase SMG-1; serine/threonine-protein kinase SMG1; SMG-1; smg-1 homolog, phosphatidylinositol 3-kinase-related kinase; SMG1 phosphatidylinositol 3-kinase-related kinase

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.