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Rabbit anti-Caf1p150 Antibody

Bethyl Laboratories®
Bethyl Laboratories®

Catalog #

Validated
Target:

Caf1p150

Reactivity:

Human

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

between 500 and 550

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (200 µg/ml)

10 µl (200 µg/ml)

Product Details

Specifications
Verified Reactivity

Human

Antigen Species

Human

Concentration
200 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide


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Additional Product Information

Caf1p150 is one of three subunits that make up the chromatin assembly factor I (CAFI). The CAFI subunits include Caf1 (p150; CHAF1A), P50, and p60 (CHAF1B; MIM 601245). The CAFI complex plays an important role in histone octamer assembly during DNA replication. The p150 subunit has been shown to directly interact with histone H3 and H4 as well as the proliferating cell nuclear antigen (PCNA) and is critical to nucleosome assembly during DNA replication.

10036

CHAF1A

chromatin assembly factor 1 subunit A

Q13111

Chromatin assembly factor 1 subunit A

Alternate Names

CAF1; CAF-1; CAF-1 subunit A; CAF1B; CAF1P150; CAF-I 150 kDa subunit; CAF-I p150; chromatin assembly factor 1 subunit A; chromatin assembly factor I (150 kDa); chromatin assembly factor I p150 subunit; CTB-50L17.7; hp150; P150

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.