/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-eIF4E Antibody
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Primary Antibodies  

Rabbit anti-eIF4E Antibody

Bethyl Laboratories®
Bethyl Laboratories®

Catalog #

Validated
Citations (12)
Target:

eIF4E

Reactivity:

Human

,

Mouse

Applications:

IP

,

WB

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

between 167 and 217

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (200 µg/ml)

10 µl (200 µg/ml)

Product Details

Specifications
Verified Reactivity

Human,

Mouse

Presumed Reactivity

Rabbit,

X_laevis,

Bovine,

Rat

Antigen Species

Human

Concentration
200 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide


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Additional Product Information

eIF4E is a component of the EIF4F initiation complex (eIF4A, eIF4E, eIF4G) that is involved in the processes of mRNA cap recognition, poly (A) binding, and the recruitment of mRNA to the ribosome during protein synthesis. eIF4E plays a role in binding the 5’cap of the mRNA for translation initiation.

1977

EIF4E

eukaryotic translation initiation factor 4E

P06730

Eukaryotic translation initiation factor 4E

Alternate Names

AUTS19; CBP; eIF4E; eIF-4E; EIF4E1; EIF4EL1; EIF4F; eIF-4F 25 kDa subunit; eukaryotic translation initiation factor 4E; eukaryotic translation initiation factor 4E-like 1; mRNA cap-binding protein

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.